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KMID : 1161520010050010085
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Animal Cells and Systems
2001 Volume.5 No. 1 p.85 ~ p.90
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Utilization of a storage protein in the embryonic development of drosophila and xenopus
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Jeong Young-Eui
Chung Hae-Moon
Ahn Tae-In
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Abstract
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Yolk platelets, one of the main food stores in the embryonic development, are composed of proteins. However, little is known about the identity of proteins utilized at certain stages of embryogenesis. In this study, we followed the fates of embryonic storage proteins by using an anti?polyubiquitin monoclonal antibody (mAB) as a probe. The mAb recognized the major storage proteins of Dros op hila, Xenopus and chicken eggs. In the Drosophila embryo, the mAb?reactive 45?kDa protein was not used until stage 11 but was used up at stage 16 when the embryo completed segmentation. In the Xenopus embryo, the mAb?reactive 111 kDa protein was mostly utilized between stages 42 and 45 implying that the protein might be an energy source used just prior to feeding on food. By N?terminal sequencing the storage protein of Xenopus embryo was identified as a lipovitellin 1. This study confirms that storage proteins are used almost simultaneously at certain stages of embryogenesis and that vitellogenin 1 is the last storage protein in Xenopus embryogenesis.
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KEYWORD
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Yolk protein, Vitellogenin, Embryogenesis, Ubiquitin?conjugated protein
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